Search results for " Closteroviridae"

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Epidemiological Survey of Grapevine Leafroll-Associated Virus 1 and 3 in Sicily (Italy): Genetic Structure and Molecular Variability

2022

Background: the most widely distributed and virulent Grapevine leafroll-associated viruses (GLRaV) that affect grapevine are GLRaV-1 and GLRaV-3, transmitted semi-persistently by different mealybugs and soft scales, mainly causing downward rolling of the leaf margins and interveinal reddening. Methods: the main objectives of this study were to investigate the genetic structure and molecular diversity of GLRaV-1 and GLRaV-3 in 617 samples from 11 autochthonous Sicilian grapevine cultivars, ascertaining their presence and spread. The detection was implemented by serological and molecular analyses and subsequently phylogenetic analyses on selected Sicilian isolates were conducted. Results: in …

grapevine disease; GLRaV-1; GLRaV-3; <i>Closteroviridae</i>; <i>Ampelovirus</i>; DAS-ELISA; RT-PCR; phylogenetic analysesPlant ScienceAgronomy and Crop Sciencegrapevine disease GLRaV-1 GLRaV-3 Closteroviridae Ampelovirus DAS-ELISA RT-PCR phylogenetic analysesFood ScienceAgriculture; Volume 12; Issue 5; Pages: 647

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